Abstract
Rice in an immature ripening growth phase is a source of vitamin, bioactive compounds and dietary fiber such as non-starch polysaccharides (NSP). NSP from plant has been shown to have immuno-stimulatory activities. In this study, rice at 13th days after anthesis was extracted for the water soluble NSP by enzymatic gravimetric method. The NSP was isolated and purified using gel filtration column. The immuno-regulatory activity was tested on murine macrophage cell line (RAW264.7)including, morphological changes of actin cytoskeleton and expression of costimulatory molecules. The results showed that NSP did not induce cytotoxic effect. In addition, the changing of morphology of macrophage cells was detected after treated with NSP. NSP from rice could enhance the expression of costimulatory molecules (CD80 and CD86) and major histocompatibility complex molecules II (MHCII) in dose dependently manner. These results indicated that NSP from the immature rice was non-toxic and had effect on the immuno-regulatory activity on macrophage cells.
